Detection of the Lyme disease bacterium, Borrelia burgdorferi, by using the polymerase chain reaction and a nonradioisotopic gene probe.
نویسندگان
چکیده
A 419-bp region of the flagellin gene sequence of Borrelia burgdorferi was used as a target for the polymerase chain reaction. With a nonradioactively labeled gene-specific probe, sensitivity to as few as 1 to 10 spirochetes was observed. The targeted gene fragment was conserved in the American and European strains of B. burgdorferi tested and among several other pathogenic borreliae.
منابع مشابه
Detection of Borrelia burgdorferi DNA by the polymerase chain reaction.
DNA amplification by the polymerase chain reaction (PCR) was used to detect DNA of the Lyme disease spirochaete Borrelia burgdorferi. Primers that specify the amplification of a 145 basepair DNA fragment of the OspA gene of B. burgdorferi were used. The amplification product was detected by gel electrophoresis and ethidium bromide staining or by hybridization to a radiolabelled oligonucleotide ...
متن کاملIdentification of Two Epitopes on the Outer Surface Protein A of the Lyme Disease Spirochete Borrelia burgdorferi
A murine IgM monoclonal antibody (MA-2C6) with κ-light chains directed against an antigenic determinant of outer surface protein A (OspA) of the Lyme disease spirochete, Borreliaburgdorferi, is produced. This antibody could bind specifically to OspA antigen of several isolates of B. burgdorferi, but not to the non-Lyme disease bacteria such as T. pallidum and B. hermsii. Antibody MA-2C6 was pur...
متن کاملDetection of Borrelia burgdorferi using the polymerase chain reaction.
Segments of the ospA gene of Borrelia burgdorferi were amplified by the polymerase chain reaction (PCR). Oligonucleotide primers used in the reaction flank a 309-base-pair segment within the ospA gene. After 35 cycles of amplification, the product could be detected by agarose gel electrophoresis or dot hybridization with a 32P-labeled probe. This segment was amplified in all strains of B. burgd...
متن کاملDetection of Borrelia burgdorferi DNA in urine of patients with ocular Lyme borreliosis.
AIM To evaluate the diagnostic value of the polymerase chain reaction (PCR) to detect Borrelia burgdorferi DNA in patients with ocular Lyme borreliosis. METHODS Of 256 consecutive uveitis patients six selected individuals with clinical evidence for Lyme borreliosis and 30 patients with non-Lyme uveitis were enrolled. Lyme serology was performed by ELISA and western blotting. Urine samples wer...
متن کاملImproving the specificity of 16S rDNA-based polymerase chain reaction for detecting Borrelia burgdorferi sensu lato-causative agents of human Lyme disease.
AIMS 16S rDNA sequences of Borrelia burgdorferi sensu lato were aligned with the 16S rDNA sequences of Borrelia hermsii, Borrelia turicatae, and Borrelia lonestari in order to identify primers that might be used to more specifically identify agents of human Lyme disease in ticks in human skin samples. METHODS AND RESULTS Standard polymerase chain reaction (PCR), using an oligonucleotide seque...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 29 7 شماره
صفحات -
تاریخ انتشار 1991